Methods – Page 2 – Bartesaghi Lab

- Atomic Resolution Cryo-EM Structure of B-galactosidase
- Cryo-EM, Methods
- Atomic Resolution Cryo-EM Structure of B-galactosidase We report methods to account for radiation damage and local changes in defocus and image drift, enabling visualization of atomic resolution features in a cryo-EM density map of inhibitor-bound b-galactosidase, and measuring of local flexibility of the bound inhibitor using constrained molecular dynamics simulations. Structure, 26(6), 2018.
- Fundamental Limits in Multi-Image Alignment
- Cryo-EM, Methods
- Fundamental Limits in Multi-Image Alignment IEEE Trans on Signal Process, 64(21):5707-5722, 2016.
- Constrained Single-Particle Cryo-Electron Tomography
- Cryo-EM, Methods
- Protein Secondary Structure Determination by Constrained Single-Particle Cryo-Electron Tomography We propose a hybrid strategy, constrained single-particle tomography (CSPT), that combines the advantages of cryo-electron tomography and subvolume averaging with those of traditional single-particle reconstruction and projection matching refinement. Its key components are the effective manipulation of images from tomographic tilt series to successfully extract high-resolution information and correct for…
- Collaborative Framework for Alignment and Classification of Sub-Tomograms
- Cryo-EM, Methods
- A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography Journal of Structural Biology 181(2):116-27, 2013.
- Sub-Tomogram Alignment Using Spherical Harmonics
- Cryo-EM, Methods
- Classification and 3D Averaging with Missing Wedge Correction in Biological Electron Tomography Journal of Structural Biology 162(3):436-50, 2008.
- Constrained Regularization of Digital Terrain Elevation Data
- Computer Vision, Methods
- Constrained regularization of digital terrain elevation data IEEE Geoscience and Remote Sensing Letters, 3(1):59-62, 2006.
- Tracking Objects Under Severe and Total Occlusions
- Computer Vision, Methods
- Tracking of Moving Objects Under Severe and Total Occlusions IEEE International Conference on Image Processing, ICIP 2005.
- Energy-Based Segmentation of Cryo-EM Tomograms
- Cryo-EM, Methods
- An Energy-based Three-dimensional Segmentation Approach for the Quantitative Interpretation of Electron Tomograms IEEE Trans Image Process,14(9):1314-23, 2005.