CRISPR

  • Structural Basis for Virulence Activation of Francisella tularensis
    • Structural Basis for Virulence Activation of Francisella tularensis

    • Structural Basis for Virulence Activation of Francisella tularensis

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    •   Structural Basis for Virulence Activation of Francisella tularensis The bacterium Francisella tularensis (Ft) is one of the most infectious agents known. Ft virulence is controlled by a unique combination of transcription regulators: the MglA-SspA heterodimer, PigR, and the stress signal, ppGpp. MglA-SspA assembles with the σ70-associated RNAP holoenzyme (RNAPσ70), forming a virulence-specialized polymerase. These factors activate Francisella pathogenicity island…
  • DNA Targeting by a CRISPR-Cas Complex
    • DNA Targeting by a CRISPR-Cas Complex

    • DNA Targeting by a CRISPR-Cas Complex

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    •   Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex Electron-microscopy images reveal how a CRISPR system marks specific DNA sequences for destruction. Microbes use CRISPR as a defense system to fend off viruses and other invaders, and geneticists have harnessed it to alter DNA sequences in a process called gene editing. We used cryo-electron microscopy…