• Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
    • Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
    • Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
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    •   Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements The trimeric HIV-1 Envelope protein (Env) mediates viral-host cell fusion via a network of conformational transitions, with allosteric elements in each protomer orchestrating host receptor-induced exposure of the co-receptor binding site and fusion elements. To understand the molecular details of this allostery, here, we introduce Env mutations aimed to…
  • Prefusion Structure of Trimeric HIV-1
    • Prefusion Structure of Trimeric HIV-1
    • Prefusion Structure of Trimeric HIV-1
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    •   Prefusion Structure of Trimeric HIV-1 Envelope Glycoprotein Determined by Cryo-Electron Microscopy HIV-1 Env transitions from a closed to an open state upon binding to its cellular receptor. Single-particle cryo-EM analysis now reveals the closed state of the HIV-1 Env trimer at ~6-Å resolution, featuring three gp41 helices at the center of the trimer. These findings indicate that HIV-1 enters…
  • Glycoprotein Spikes on Intact HIV and SIV Viruses
    • Glycoprotein Spikes on Intact HIV and SIV Viruses
    • Glycoprotein Spikes on Intact HIV and SIV Viruses
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    •   Molecular Architectures of Trimeric SIV and HIV-1 Envelope Glycoproteins on Intact Viruses: Strain-Dependent Variation in Quaternary Structure HIV and SIV contact and infect target T-cells following the binding of trimeric Env spikes displayed on the viral membrane with cellular receptors. The conformational changes in trimeric Env that are triggered by the interaction between trimeric Env and cell surface receptors…
  • Structure of Native HIV-1 gp120 Trimers
    • Structure of Native HIV-1 gp120 Trimers
    • Structure of Native HIV-1 gp120 Trimers
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    •   Molecular Architecture of Native HIV-1 gp120 Trimers We determined the structure of the native gp120 coat protein of HIV by cryo-electron tomography and molecular modelling. Comparison of gp120 structures in an unbound state, bound to a neutralizing antibody and bound to CD4 cell surface protein provides insight into the conformational changes that occur during antibody neutralization and attachment to…