The End of ‘blob-ology’: Single-Particle Cryo-EM is Now Being Used to Solve Macromolecular Structures at High Resolution

Nature Methods, 13(1), 2015.



The choice for Method of the Year 2015 is single-particle cryo-electron microscopy. A collection of articles discusses how recent technical advances, especially the development of direct-detection cameras, have enabled this structural biology technique to make impressive leaps in achievable resolution and, in turn, provide new insights about protein function. We also highlight methods to watch in the upcoming years.