• Cryo-ZSSR: multiple-image super-resolution based on deep internal learning
    • Cryo-ZSSR: multiple-image super-resolution based on deep internal learning
    • Cryo-ZSSR: multiple-image super-resolution based on deep internal learning
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    •   Cryo-ZSSR: multiple-image super-resolution based on deep internal learning We present a multiple-image super-resolution (SR) algorithm based on deep internal learning designed specifically to work under low-SNR conditions typical of cryo-EM data. Our approach leverages the internal image statistics of cryo-EM movies and does not require training on ground-truth data. When applied to a single-particle dataset of apoferritin, we show…
  • Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
  • Single-Particle Cryo-Electron Microscopy
    • Single-Particle Cryo-Electron Microscopy
    • Single-Particle Cryo-Electron Microscopy
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    •   Mathematical Theory, Computational Challenges, and Opportunities Structural biology studies the structure and dynamics of macromolecules to broaden our knowledge about the mechanisms of life and impact the drug-discovery process. Owing to recent groundbreaking developments, chiefly in hardware technologies and data processing techniques, many new molecular structures have been elucidated to near-atomic resolutions using cryo-EM. The main goal of this article…
  • Unsupervised particle sorting for high-resolution single-particle cryo-EM
    • Unsupervised particle sorting for high-resolution single-particle cryo-EM
    • Unsupervised particle sorting for high-resolution single-particle cryo-EM
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    •   Unsupervised particle sorting for high-resolution single-particle cryo-EM Single-particle cryo-Electron Microscopy (EM) has become a popular technique for determining the structure of challenging biomolecules that are inaccessible to other technologies. Recent advances in automation, both in data collection and data processing, have significantly lowered the barrier for non-expert users to successfully execute the structure determination workflow. Many critical data processing…
  • Atomic Resolution Cryo-EM Structure of B-galactosidase
    • Atomic Resolution Cryo-EM Structure of B-galactosidase
    • Atomic Resolution Cryo-EM Structure of B-galactosidase
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    •   Atomic Resolution Cryo-EM Structure of B-galactosidase We report methods to account for radiation damage and local changes in defocus and image drift, enabling visualization of atomic resolution features in a cryo-EM density map of inhibitor-bound b-galactosidase, and measuring of local flexibility of the bound inhibitor using constrained molecular dynamics simulations. Structure, 26(6), 2018.
  • Fundamental Limits in Multi-Image Alignment
  • Constrained Single-Particle Cryo-Electron Tomography
    • Constrained Single-Particle Cryo-Electron Tomography
    • Constrained Single-Particle Cryo-Electron Tomography
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    •   Protein Secondary Structure Determination by Constrained Single-Particle Cryo-Electron Tomography We propose a hybrid strategy, constrained single-particle tomography (CSPT), that combines the advantages of cryo-electron tomography and subvolume averaging with those of traditional single-particle reconstruction and projection matching refinement. Its key components are the effective manipulation of images from tomographic tilt series to successfully extract high-resolution information and correct for…
  • Collaborative Framework for Alignment and Classification of Sub-Tomograms