Publications – Page 6 – Bartesaghi Lab

- Prefusion Structure of Trimeric HIV-1
- HIV, Membrane Proteins, Publications, Structures
- Prefusion Structure of Trimeric HIV-1 Envelope Glycoprotein Determined by Cryo-Electron Microscopy HIV-1 Env transitions from a closed to an open state upon binding to its cellular receptor. Single-particle cryo-EM analysis now reveals the closed state of the HIV-1 Env trimer at ~6-Å resolution, featuring three gp41 helices at the center of the trimer. These findings indicate that HIV-1 enters…
- Structure of Native HIV-1 gp120 Trimers
- HIV, Membrane Proteins, Publications, Structures
- Molecular Architecture of Native HIV-1 gp120 Trimers We determined the structure of the native gp120 coat protein of HIV by cryo-electron tomography and molecular modelling. Comparison of gp120 structures in an unbound state, bound to a neutralizing antibody and bound to CD4 cell surface protein provides insight into the conformational changes that occur during antibody neutralization and attachment to…
- Constrained Single-Particle Cryo-Electron Tomography
- Cryo-EM, Methods, Publications
- Protein Secondary Structure Determination by Constrained Single-Particle Cryo-Electron Tomography We propose a hybrid strategy, constrained single-particle tomography (CSPT), that combines the advantages of cryo-electron tomography and subvolume averaging with those of traditional single-particle reconstruction and projection matching refinement. Its key components are the effective manipulation of images from tomographic tilt series to successfully extract high-resolution information and correct for…
- Collaborative Framework for Alignment and Classification of Sub-Tomograms
- Cryo-EM, Methods, Publications
- A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography Journal of Structural Biology 181(2):116-27, 2013.
- Sub-Tomogram Alignment Using Spherical Harmonics
- Cryo-EM, Methods, Publications
- Classification and 3D Averaging with Missing Wedge Correction in Biological Electron Tomography Journal of Structural Biology 162(3):436-50, 2008.
- Constrained Regularization of Digital Terrain Elevation Data
- Computer Vision, Methods, Publications
- Constrained regularization of digital terrain elevation data IEEE Geoscience and Remote Sensing Letters, 3(1):59-62, 2006.
- Tracking Objects Under Severe and Total Occlusions
- Computer Vision, Methods, Publications
- Tracking of Moving Objects Under Severe and Total Occlusions IEEE International Conference on Image Processing, ICIP 2005.
- Energy-Based Segmentation of Cryo-EM Tomograms
- Cryo-EM, Methods, Publications
- An Energy-based Three-dimensional Segmentation Approach for the Quantitative Interpretation of Electron Tomograms IEEE Trans Image Process,14(9):1314-23, 2005.