• Redox-sensitive E2 Rad6 controls cellular response to oxidative stress via K63-linked ubiquitination of ribosomes
  • Structural basis of NPR1 in activating plant immunity
    • Structural basis of NPR1 in activating plant immunity
    • Structural basis of NPR1 in activating plant immunity
    •   Structural basis of NPR1 in activating plant immunity We report cryo-electron microscopy and crystal structures of Arabidopsis NPR1 and its complex with the transcription factor TGA3. Cryo-electron microscopy analysis reveals that NPR1 is a bird-shaped homodimer comprising a central Broad-complex, Tramtrack and Bric-à-brac (BTB) domain, a BTB and carboxyterminal Kelch helix bundle, four ankyrin repeats and a disordered salicylic-acid-binding…
  • Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies
  • Structural Basis for Virulence Activation of Francisella tularensis
    • Structural Basis for Virulence Activation of Francisella tularensis
    • Structural Basis for Virulence Activation of Francisella tularensis
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    •   Structural Basis for Virulence Activation of Francisella tularensis The bacterium Francisella tularensis (Ft) is one of the most infectious agents known. Ft virulence is controlled by a unique combination of transcription regulators: the MglA-SspA heterodimer, PigR, and the stress signal, ppGpp. MglA-SspA assembles with the σ70-associated RNAP holoenzyme (RNAPσ70), forming a virulence-specialized polymerase. These factors activate Francisella pathogenicity island…
  • Structural Impact of K63 Ubiquitin
    • Structural Impact of K63 Ubiquitin
    • Structural Impact of K63 Ubiquitin
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    •   Structural impact of K63 ubiquitin on yeast translocating ribosomes under oxidative stress K63 ubiquitination of ribosomes serves as a key regulator of protein production during cellular exposure to oxidative stress. Defining the structural and functional mechanisms of translation regulation would support the current understanding of critical reprogramming of eukaryotic gene expression. Our paper presents an examination of the structure…
  • Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
    • Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
    • Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
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    •   Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements The trimeric HIV-1 Envelope protein (Env) mediates viral-host cell fusion via a network of conformational transitions, with allosteric elements in each protomer orchestrating host receptor-induced exposure of the co-receptor binding site and fusion elements. To understand the molecular details of this allostery, here, we introduce Env mutations aimed to…
  • Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs
  • Structure of rhodopsin bound to an inhibitory G protein
    • Structure of rhodopsin bound to an inhibitory G protein
    • Structure of rhodopsin bound to an inhibitory G protein
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    •   Cryo-EM structure of human rhodopsin bound to an inhibitory G protein For the first time, scientists have visualized the interaction between two critical components of the body’s vast cellular communication network, a discovery that could lead to more effective medications with fewer side effects for conditions ranging from migraine to cancer. The near-atomic resolution images obtained, show a G-protein coupled…