• nextPYP: a comprehensive and scalable platform for single-particle cryo-ET
  • Molecular architecture and conservation of an immature human endogenous retrovirus
    • Molecular architecture and conservation of an immature human endogenous retrovirus
    • Molecular architecture and conservation of an immature human endogenous retrovirus

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    •   Molecular architecture and conservation of an immature human endogenous retrovirus The human endogenous retrovirus K (HERV-K) is the most recently acquired endogenous retrovirus in the human genome and is activated and expressed in many cancers and amyotrophic lateral sclerosis. We present the immature HERV-K capsid structure at 3.2 Å resolution determined from native virus-like particles using cryo-electron tomography and subtomogram…
  • Structural basis for breadth development in the HIV-1 V3-glycan targeting DH270 antibody clonal lineage
  • Structure and dynamics of the Arabidopsis O-fucosyltransferase SPINDLY
    • Structure and dynamics of the Arabidopsis O-fucosyltransferase SPINDLY
    • Structure and dynamics of the Arabidopsis O-fucosyltransferase SPINDLY

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    •   Structure and dynamics of the Arabidopsis O-fucosyltransferase SPINDLY SPINDLY (SPY) in Arabidopsis thaliana is a novel nucleocytoplasmic protein O-fucosyltransferase (POFUT), which regulates diverse developmental processes. Sequence analysis indicates that SPY is distinct from ER-localized POFUTs and contains N-terminal tetratricopeptide repeats (TPRs) and a C-terminal catalytic domain resembling the O-linked-N-acetylglucosamine (GlcNAc) transferases (OGTs). However, the structural feature that determines the…
  • Redox-sensitive E2 Rad6 controls cellular response to oxidative stress via K63-linked ubiquitination of ribosomes
  • Structural basis of NPR1 in activating plant immunity
    • Structural basis of NPR1 in activating plant immunity
    • Structural basis of NPR1 in activating plant immunity

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    •   Structural basis of NPR1 in activating plant immunity We report cryo-electron microscopy and crystal structures of Arabidopsis NPR1 and its complex with the transcription factor TGA3. Cryo-electron microscopy analysis reveals that NPR1 is a bird-shaped homodimer comprising a central Broad-complex, Tramtrack and Bric-à-brac (BTB) domain, a BTB and carboxyterminal Kelch helix bundle, four ankyrin repeats and a disordered salicylic-acid-binding…
  • Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies
  • Structural Basis for Virulence Activation of Francisella tularensis
    • Structural Basis for Virulence Activation of Francisella tularensis
    • Structural Basis for Virulence Activation of Francisella tularensis

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    •   Structural Basis for Virulence Activation of Francisella tularensis The bacterium Francisella tularensis (Ft) is one of the most infectious agents known. Ft virulence is controlled by a unique combination of transcription regulators: the MglA-SspA heterodimer, PigR, and the stress signal, ppGpp. MglA-SspA assembles with the σ70-associated RNAP holoenzyme (RNAPσ70), forming a virulence-specialized polymerase. These factors activate Francisella pathogenicity island…