• Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs
  • Structure of rhodopsin bound to an inhibitory G protein
    • Structure of rhodopsin bound to an inhibitory G protein
    • Structure of rhodopsin bound to an inhibitory G protein
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    •   Cryo-EM structure of human rhodopsin bound to an inhibitory G protein For the first time, scientists have visualized the interaction between two critical components of the body’s vast cellular communication network, a discovery that could lead to more effective medications with fewer side effects for conditions ranging from migraine to cancer. The near-atomic resolution images obtained, show a G-protein coupled…
  • Atomic Resolution Structure of B-galactosidase
    • Atomic Resolution Structure of B-galactosidase
    • Atomic Resolution Structure of B-galactosidase
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    •   Atomic Resolution Cryo-EM Structure of B-galactosidase We report methods to account for radiation damage and local changes in defocus and image drift, enabling visualization of atomic resolution features in a cryo-EM density map of inhibitor-bound -galactosidase, and derivation of atom-specific measures of local flexibility of the bound inhibitor using constrained molecular dynamics simulations. Structure, 26(6), p. 848-85, 2018.
  • DNA Targeting by a CRISPR-Cas Complex
    • DNA Targeting by a CRISPR-Cas Complex
    • DNA Targeting by a CRISPR-Cas Complex
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    •   Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex Electron-microscopy images reveal how a CRISPR system marks specific DNA sequences for destruction. Microbes use CRISPR as a defense system to fend off viruses and other invaders, and geneticists have harnessed it to alter DNA sequences in a process called gene editing. We used cryo-electron microscopy…
  • Proteins Involved in Cancer Cell Metabolism
    • Proteins Involved in Cancer Cell Metabolism
    • Proteins Involved in Cancer Cell Metabolism
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    •   Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery Using cryo-EM we were able to capture images of glutamate dehydrogenase (GDH), an enzyme found in cells, at a resolution of 1.8 angstroms, a level of detail at which the structure of the central parts of the enzyme could be visualized in atomic detail. We also imaged two small proteins in…
  • Human p97 and Mechanism of Allosteric Inhibition
    • Human p97 and Mechanism of Allosteric Inhibition
    • Human p97 and Mechanism of Allosteric Inhibition
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    •   2.3 Å Resolution Cryo-EM Structure of Human p97 and Mechanism of Allosteric Inhibition The protein p97 is an AAA adenosine triphosphatase (ATPase) that uses energy from ATP hydrolysis to regulate substrates involved in intracellular protein quality control. Its role in this central process makes it a target for cancer chemotherapy. We used cryo-electron microscopy to determine high-resolution structures for…
  • Mapping Small Ligands on Dynamic Metabolic Enzymes
  • Cryo-EM Analysis of Human P-glycoprotein